Anti-FIF antibody (ab65836)
Key features and details
- Rabbit polyclonal to FIF
- Suitable for: ICC/IF, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FIF antibody
See all FIF primary antibodies -
Description
Rabbit polyclonal to FIF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-Fr RatIHC-P MouseRatHumanWB Rat
-
Immunogen
Synthetic peptide corresponding to Human FIF.
Database link: Q9BZZ5 -
Positive control
- WB: Rat Cardiac Muscle, Rat Brain, Rat Testis, Rat Placenta, MCF-7, HeLa, CEM, SMMC, and COLO320 Lysates. IHC-P: Rat Brain, Human placenta, Rat intestines, and Mouse intestines testis. IHC-F: Rat Cardiac Muscle. ICC/IF: HeLa cells.
-
General notes
Previously labelled as Apoptosis inhibitor 5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat intestine tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
-
Western blot - Anti-FIF antibody (ab65836)All lanes : Anti-FIF antibody (ab65836) at 0.5 µg/ml
Lane 1 : Rat Cardiac Muscle Tissue Lysate
Lane 2 : Rat Brain Tissue Lysate
Lane 3 : Rat Testis Tissue Lysate
Lane 4 : Rat Placenta Tissue Lysate
Lane 5 : MCF-7 Cell Lysate
Lane 6 : HeLa Cell Lysate
Lane 7 : CEM Cell Lysate
Lane 8 : SMMC Cell Lysate
Lane 9 : COLO320 Cell Lysate
Predicted band size: 58 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta tissue staining FIF using ab65836 at 1 μg/ml.
Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
-
Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)Immunocytochemistry/ Immunofluorescence of HeLa cells staining FIF using ab65836 at 1 μg/ml.
-
Immunohistochemistry (Frozen sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Frozen sections) of Rat cardiac muscel tissue staining FIF using ab65836 at 1 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue staining FIF using ab65836 at 1 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab65836)This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (ab65836)ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
