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Anti-NFkB p100/NFKB2 antibody (ab131539)

Anti-NFkB p100/NFKB2 antibody (ab131539)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NFkB p100/NFKB2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NFkB p100/NFKB2 antibody
    See all NFkB p100/NFKB2 primary antibodies
  • Description

    Rabbit polyclonal to NFkB p100/NFKB2
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous level of total NFkB-p100 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human NFkB p100/NFKB2 aa 870-874 conjugated to keyhole limpet haemocyanin.
    Sequence:

    S-Q-S-V-E


    Database link: Q00653
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; HT29 cell extract; Human lung carcinoma tissue
  • General notes

    This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.

     This product was previously labelled as NFkB p100

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • p52
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • p52

Images

  • Western blot - Anti-NFkB p100/NFKB2 antibody (ab131539)
    Western blot - Anti-NFkB p100/NFKB2 antibody (ab131539)
    All lanes : Anti-NFkB p100/NFKB2 antibody (ab131539) at 1/500 dilution

    Lane 1 : HT29 cell extract with blocking peptide
    Lane 2 : HT29 cell extract

    Predicted band size: 97 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab131539)
    Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab131539)

    Immunofluorescence analysis of methanol-fixed HeLa cells labelling NFkB p100/NFKB2 with ab131539 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling NFkB p100/NFKB2 with ab131539 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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