Anti-NFkB p100/NFKB2 antibody (ab131539)
Key features and details
- Rabbit polyclonal to NFkB p100/NFKB2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p100/NFKB2 antibody
See all NFkB p100/NFKB2 primary antibodies -
Description
Rabbit polyclonal to NFkB p100/NFKB2 -
Host species
Rabbit -
Specificity
The antibody detects endogenous level of total NFkB-p100 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human NFkB p100/NFKB2 aa 870-874 conjugated to keyhole limpet haemocyanin.
Sequence:S-Q-S-V-E
Database link: Q00653 -
Positive control
- HeLa cells; HT29 cell extract; Human lung carcinoma tissue
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General notes
This antibody detects sites on p100 only. Sometimes there is a 52kDa band on WB; this is not p52. There is a cleavage site on the NFKB2 (Swiss-Prot No.: Q00653). The cleavage site is between 454 and 455 (cleavage when cotranslationally processed). The antibody can interact with the C-half sometimes. The MW. of C-half is near 52Kd.
This product was previously labelled as NFkB p100
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NFkB p100/NFKB2 antibody (ab131539) at 1/500 dilution
Lane 1 : HT29 cell extract with blocking peptide
Lane 2 : HT29 cell extract
Predicted band size: 97 kDa
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Immunofluorescence analysis of methanol-fixed HeLa cells labelling NFkB p100/NFKB2 with ab131539 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab131539)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling NFkB p100/NFKB2 with ab131539 at 1/50 dilution. Right panel was preincubated with blocking peptide.