Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)

Key features and details

  • Mouse monoclonal [BBmLBR 12.F8] to Lamin B Receptor/LBR - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free
    See all Lamin B Receptor/LBR primary antibodies

  • Description

    Mouse monoclonal [BBmLBR 12.F8] to Lamin B Receptor/LBR - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293 cells. WB: MEF-1 whole cell lysate IHC-P: Human duodenum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269576 is the carrier-free version of ab232731. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    Western blot - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    All lanes : Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] (ab232731) at 1 µg/ml

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : Human LBR (Lamin B Receptor) knockout HEK-293T cell lysate (ab257503)
    Lane 3 : Wild-type MEF-1 whole cell lysate
    Lane 4 : LBR knockout MEF-1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab232731).

    Lanes 1 - 4: Merged signal (red and green). Green - ab232731 observed at 58 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

    ab232731 was shown to react with Lamin B Receptor (LBR) in wild-type HEK-293 and MEF-1 cells in western blot. Loss of signal was observed when LBR knockout samples were used. Wild-type and LBR knockout HEK-293 cell lysates (ab257503) and wild-type and LBR knockout MEF-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab232731 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    MEF-1 LBR knockout samples were kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)

    ab232731 staining Lamin B Receptor in HEK-293 cells. The cells were fixed with 4% paraformaldehyde (10min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232731 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was produced using the same antibody clone but in a different formulation ab232731, PBS and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)

    IHC image of Lamin B receptor staining in a section of formalin-fixed paraffin-embedded normal human duodenum* performed on a Leica BondTM system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab232731, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    This image was produced using the same antibody clone but in a different formulation ab232731, PBS and sodium azide.

  • Western blot - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    Western blot - Anti-Lamin B Receptor/LBR antibody [BBmLBR 12.F8] - BSA and Azide free (ab269576)
    All lanes : Anti-Histone H4 antibody [EPR16599] - BSA and Azide free (ab232371) at 1 µg/ml

    Lane 1 : MEF-1 wild-type whole cell lysate
    Lane 2 : MEF-1 LBR null knockout whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 71 kDa



    This image was produced using the same antibody clone but in a different formulation ab232731, PBS and sodium azide.

    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab232731 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.

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