Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9197(B)] to TAF15
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TAF15 antibody [EPR9197(B)]
    See all TAF15 primary antibodies

  • Description

    Rabbit monoclonal [EPR9197(B)] to TAF15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TAF15 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q92804

  • Positive control

    • HeLa, 293T, Caco 2, and Human fetal kidney lysates; Human urinary bladder carcinoma tissue; HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Western blot - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    All lanes : Anti-TAF15 antibody [EPR9197(B)] (ab134916) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

    ab134916 staining TAF15 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Flow Cytometry - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Flow Cytometry - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Overlay histogram showing HeLa cells stained with ab134916 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134916, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

    Immunohistochemical analysis of TAF15 in paraffin embedded Human urinary bladder carcinoma tissue, using ab134916 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Immunofluorescent analysis of TAF15 in HeLa cells, using ab134916 at a 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (ab134916)

    Immunohistochemical analysis using ab134916 showing positive staining in Normal human uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TAF15 antibody [EPR9197(B)] (ab134916)
    Anti-TAF15 antibody [EPR9197(B)] (ab134916)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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