Anti-TAF15 antibody (ab237809)
Key features and details
- Rabbit polyclonal to TAF15
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TAF15 antibody
See all TAF15 primary antibodies -
Description
Rabbit polyclonal to TAF15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Recombinant fragment corresponding to Human TAF15 aa 93-206.
Database link: Q92804 -
Positive control
- IHC-P: Human testis tissue. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody (ab237809)
Paraffin-embedded human testis tissue stained for TAF15 using ab237809 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody (ab237809)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TAF15 (green) using ab237809 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
