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Anti-5HT4 Receptor antibody (ab60359)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-5HT4 Receptor antibody (ab60359)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to 5HT4 Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, Zebrafish
  • Isotype: IgG

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Overview

  • Product name

    Anti-5HT4 Receptor antibody
    See all 5HT4 Receptor primary antibodies
  • Description

    Rabbit polyclonal to 5HT4 Receptor
  • Host species

    Rabbit
  • Specificity

    5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human 5HT4 Receptor aa 21-70.
    Sequence:

    LMAILGNLLV MVAVCWDRQL RKIKTNYFIV SLAFADLLVS


    Database link: Q13639
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Jurkat cell, COLO205 cells. ICC: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Serotonin Receptors
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-5HT4 Receptor antibody (ab60359)
    Western blot - Anti-5HT4 Receptor antibody (ab60359)
    All lanes : Anti-5HT4 Receptor antibody (ab60359)

    Lane 1 : Jurkat cell lysate
    Lane 2 : COLO205 cell lysate
    Lane 3 : COLO205 cell lysate with Synthesized peptide

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (ab60359)
    Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (ab60359)
    ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right).
  • Western blot - Anti-5HT4 Receptor antibody (ab60359)
    Western blot - Anti-5HT4 Receptor antibody (ab60359)
    Anti-5HT4 Receptor antibody (ab60359) at 1/1000 dilution + COLO205 cells

    Predicted band size: 44 kDa

  • Western blot - Anti-5HT4 Receptor antibody (ab60359)
    Western blot - Anti-5HT4 Receptor antibody (ab60359)
    All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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