Mouse Endocan ELISA Kit (ab193722)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.1 ng/ml - 30 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Endocan ELISA Kit
See all ESM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.1 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 123.8 108% - 149% Plasma 121.1 108% - 146% Cell culture media 103.8 85% - 122% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse
Does not react with: Human -
Product overview
Abcam's Endocan Mouse ELISA Kit (ab193722) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Endocan in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Endocan coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Endocan present in the samples. The wells are washed and biotinylated anti-mouse Endocan antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Endocan bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated mouse Endocan detection antibody (lyophilized) 2 vials mouse Endocan Standards (lyophilized) 2 vials Pre-coated mouse Endocan Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May have potent implications in lung endothelial cell-leukocyte interactions. -
Tissue specificity
Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney. -
Sequence similarities
Contains 1 IGFBP N-terminal domain. -
Post-translational
modificationsMay contain intrachain disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Endocan
- Endothelial cell specific molecule 1
- Endothelial cell-specific molecule 1
see all -
Database links
- Entrez Gene: 71690 Mouse
- SwissProt: Q9QYY7 Mouse
- Unigene: 38929 Mouse
Images
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Standard Curve.
Typical Standard Curve using ab193722 Endocan Mouse ELISA Kit.
