Mouse Endocan ELISA Kit (ab236716)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Endocan ELISA Kit
See all ESM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.9% Inter-assay Sample n Mean SD CV% Serum 3 10.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117 117% - 117% Cell culture media 84 82% - 85% Hep Plasma 110 103% - 115% EDTA Plasma 113 111% - 116% Cit plasma 109 108% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Endocan ELISA Kit (ab236716) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endocan protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Endocan with 9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Endocan Capture Antibody 1 x 600µl 10X Mouse Endocan Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Endocan Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May have potent implications in lung endothelial cell-leukocyte interactions. -
Tissue specificity
Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney. -
Sequence similarities
Contains 1 IGFBP N-terminal domain. -
Post-translational
modificationsMay contain intrachain disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Endocan
- Endothelial cell specific molecule 1
- Endothelial cell-specific molecule 1
see all -
Database links
- Entrez Gene: 71690 Mouse
- SwissProt: Q9QYY7 Mouse
- Unigene: 38929 Mouse
Images
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Other - Mouse Endocan ELISA Kit (ab236716)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Competitor Curve ComparisonStandard curve comparison between mouse Endocan SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse Endocan standard curve in Sample Diluent NS.The Endocan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Endocan in mouse serum and plasma samples.The concentrations of Endocan were measured in duplicates, interpolated from the Endocan standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (EDTA) 20%, and plasma (heparin) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endocan concentration was determined to be 6095 pg/mL in serum, 6649 pg/mL in plasma (citrate), 5733 pg/mL in plasma (EDTA) and 5509 pg/mL in plasma (heparin).
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Interpolated concentrations of spike Endocan in mouse cell culture media sample.The concentrations of Endocan were measured in duplicates, interpolated from the Endocan standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Mouse Endocan ELISA Kit (ab236716)To learn more about the advantages of recombinant antibodies see here.
