Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD55 antibody [EPR6689] (ab133684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6689] to CD55
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD55 antibody [EPR6689]
    See all CD55 primary antibodies

  • Description

    Rabbit monoclonal [EPR6689] to CD55

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control

    • WB: A549, and HeLa lysates. IHC-P: human cervix carcinoma, lung and placenta tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.25 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6689

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue sections labeling CD55 with purified ab133684 at 1/4000 dilution (0.04 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CD55 antibody [EPR6689] (ab133684)
    Western blot - Anti-CD55 antibody [EPR6689] (ab133684)
    All lanes : Anti-CD55 antibody [EPR6689] (ab133684) at 1/15000 dilution (Purified)

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 70-75 kDa
    why is the actual band size different from the predicted?



    CD55 is a 70- to 75-kDa GPI-anchored membrane protein (PMID: 8764022)

  • Western blot - Anti-CD55 antibody [EPR6689] (ab133684)
    Western blot - Anti-CD55 antibody [EPR6689] (ab133684)
    All lanes : Anti-CD55 antibody [EPR6689] (ab133684) at 1/10000 dilution ((unpurified))

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with unpurified ab133684 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (ab133684)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD55 with unpurified ab133684 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD55 antibody [EPR6689] (ab133684)
    OI-RD Scanning - Anti-CD55 antibody [EPR6689] (ab133684)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD55 antibody [EPR6689] (ab133684)
    Anti-CD55 antibody [EPR6689] (ab133684)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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