Anti-beta Galactosidase antibody (ab134435)
Key features and details
- Chicken polyclonal to beta Galactosidase
- Suitable for: IHC-FoFr, ICC/IF
- Isotype: IgY
Overview
-
Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Chicken polyclonal to beta Galactosidase -
Host species
Chicken -
Tested applications
Suitable for: IHC-FoFr, ICC/IFmore details -
Immunogen
Purified beta Galactosidase (lacZ) protein emulsified in Freund's adjuvants.
-
Positive control
- Tissues infected with a virus bearing the LacZ gene
-
General notes
Do not freeze these antibodies at –80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
IgY fractions were prepared from eggs yolks, and then affinity-purified antibodies were prepared using beta Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (ab134435)
Immunohistochemical analysis of tissue infected with a virus bearing the LacZ gene labelling beta Galactosidase with ab134435 at 1/5000 dilution with e18 mouse otic vesicle. The green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).
