Daidzein, Phytoestrogen (ab120391)
Key features and details
- Phytoestrogen
- CAS Number: 486-66-8
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Daidzein, Phytoestrogen -
Description
Phytoestrogen -
Biological description
Phytoestrogen, and soy isoflavone. Analog of genistein (ab120112). Various biological actions, including a weak estrogenic / antiestrogenic effect by binding to the nuclear estrogen receptor.
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Purity
> 99% -
CAS Number
486-66-8 -
Chemical structure
Images
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Functional Studies - Daidzein, Phytoestrogen (ab120391)ab47489 staining Bcl2 MCF7 cells treated with diadzein (ab120391), by ICC/IF. Decrease in Bcl2 expression correlates with increased concentration of diadzein, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120391 (diadzein) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab47489 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
