Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µg 268 032 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Product name

Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies
Description

Rabbit polyclonal to beta Galactosidase
Host species

Rabbit
Specificity

Detects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Escherichia coli
Immunogen

Full length native protein (purified) corresponding to Escherichia coli beta Galactosidase.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

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Purity

IgG fraction
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab4761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).

Notes
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).

Relevance

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Cellular localization

Cytoplasmic
Database links
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
- ECK0341 antibody
- JW0335 antibody
- Lactase antibody
- lacZ antibody
see all

Publishing research using ab4761? Please let us know so that we can cite the reference in this datasheet.

ab4761 has been referenced in 14 publications.

Meng F et al. Brain-derived neurotrophic factor in 5-HT neurons regulates susceptibility to depression-related behaviors induced by subchronic unpredictable stress. J Psychiatr Res 126:55-66 (2020). PubMed: 32416387
Liu R et al. DDP-resistant ovarian cancer cells-derived exosomal microRNA-30a-5p reduces the resistance of ovarian cancer cells to DDP. Open Biol 10:190173 (2020). PubMed: 32343928
Khadilkar RJ & Tanentzapf G Septate junction components control Drosophila hematopoiesis through the Hippo pathway. Development 146:N/A (2019). PubMed: 30890573
Debeuf N et al. The ORMDL3 asthma susceptibility gene regulates systemic ceramide levels without altering key asthma features in mice. J Allergy Clin Immunol 144:1648-1659.e9 (2019). PubMed: 31330218
Clemente C et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). PubMed: 29500407