Anti-beta Galactosidase antibody (ab221199)
Key features and details
- Rabbit polyclonal to beta Galactosidase
- Suitable for: WB, IP, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Rabbit polyclonal to beta Galactosidase -
Host species
Rabbit -
Specificity
This antibody recognizes beta Galactosidase from E coli and beta Galactosidase tagged proteins. -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli beta Galactosidase aa 1 to the C-terminus.
Database link: B7UJI9 -
Positive control
- Purified beta Galactosidase; IPTG induced E.coli K12 cell extract; HEK293A cells transfected with beta Galactosidase cDNA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of HEK293A cells transfected with beta Galactosidase cDNA (4% PFA-fixed, methanol permeabilized) labeling beta Galactosidase with ab221199 at 1/500 dilution, followed by secondary detection with Alexa Fluor 555 conjugated anti rabbit IgG at 1/500 dilution (red). Hoecst 33342 was used for nuclear counterstaining (blue).
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All lanes : Anti-beta Galactosidase antibody (ab221199) at 1/1000 dilution
Lane 1 : purified beta Galactosidase at 0.01 µg
Lane 2 : uninduced E coli cell extract at 30 µg
Lane 3 : ITPG induced E coli cell extract at 30 µg
Predicted band size: 116 kDa
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beta Galactosidase was immunorecipitated from ITPG induced E coli crude extracts using ab221199 at 1/500 dilution.
Western blot detection was performed using ab221199 at 1/1000 dilution.
Lane 1: Crude extract
Lane 2: Supernatant of the immunoprecipitated E. coli crude extract
Lane 3: Immunoprecipitate of crude E. coli extract