Anti-beta Galactosidase antibody (ab616)
Key features and details
- Rabbit polyclonal to beta Galactosidase
- Suitable for: ELISA, WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
-
Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Rabbit polyclonal to beta Galactosidase -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IPmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein (E. coli).
-
General notes
Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-beta Galactosidase antibody (ab616)All lanes : Anti-beta Galactosidase antibody (ab616)
Lanes 1-2 : Non-reduced purified recombinant beta Galactosidase protein
Lanes 3-4 : reduced purified recombinant beta Galactosidase proteinWestern blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.
