Anti-CLASP1 antibody [KT67] (ab95372)
![Anti-CLASP1 antibody [KT67] (ab95372)](https://www.abcam.com/ps/products/95/ab95372/Images/ab95372-312098-Ab95372KOWB.jpg "Anti-CLASP1 antibody [KT67] (ab95372)")
Key features and details
- Rat monoclonal [KT67] to CLASP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CLASP1 antibody [KT67]
See all CLASP1 primary antibodies -
Description
Rat monoclonal [KT67] to CLASP1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Fusion protein corresponding to Human CLASP1 (N terminal).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KT67 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-CLASP1 antibody [KT67] (ab95372)All lanes : Anti-CLASP1 antibody [KT67] (ab95372) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLASP1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 169 kDaLanes 1 - 4: Merged signal (red and green). Green - ab95372 observed at 169 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab95372 was shown to specifically react with CLASP1 in wild-type HAP1 cells as signal was lost in CLASP1 knockout cells. Wild-type and CLASP1 knockout samples were subjected to SDS-PAGE. Ab95372 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
