Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLASP1 antibody [KT67] (ab95372)

Key features and details

  • Rat monoclonal [KT67] to CLASP1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2a

Overview

Properties

Images

  • Western blot - Anti-CLASP1 antibody [KT67] (ab95372)
    Western blot - Anti-CLASP1 antibody [KT67] (ab95372)
    All lanes : Anti-CLASP1 antibody [KT67] (ab95372) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CLASP1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 169 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab95372 observed at 169 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab95372 was shown to specifically react with CLASP1 in wild-type HAP1 cells as signal was lost in CLASP1 knockout cells. Wild-type and CLASP1 knockout samples were subjected to SDS-PAGE. Ab95372 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CLASP1 antibody [KT67] (ab95372)

© 2021 Astana Biomed Group LLP. All rights reserved.