Anti-RRAS antibody (ab191791)
Key features and details
- Rabbit polyclonal to RRAS
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RRAS antibody
See all RRAS primary antibodies -
Description
Rabbit polyclonal to RRAS -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney, rat heart and mouse spleen lysates. IP: Human fetal kidney lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RRAS antibody (ab191791) at 1/500 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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RRAS was immunoprecipitated from Human fetal kidney lysate with ab191791 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab191791 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney lysate 10 µg (Input). Lane 2: ab191791 IP in Human fetal kidney lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191791 in Human fetal kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
RRAS was immunoprecipitated from Human fetal kidney lysate with ab191791 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab191399 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney lysate 10 µg (Input). Lane 2: ab191791 IP in Human fetal kidney lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191791 in Human fetal kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.ab191791 was used to Immunoprecipitate RRAS. These precipitates were resolved with ab191399.