Anti-RRAS antibody (ab82356)
Key features and details
- Rabbit polyclonal to RRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RRAS antibody
See all RRAS primary antibodies -
Description
Rabbit polyclonal to RRAS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human RRAS aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93071) -
Positive control
- Recombinant Human RRAS protein (ab114612) can be used as a positive control in WB. This antibody gave a positive signal in human skeletal muscle as well as the following whole cell lysates: HeLa; HT29; DU145; PC3; A549.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Notes Application notesWB: 1/250. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Regulates the organization of the actin cytoskeleton. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Cellular localization
Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine. - Information by UniProt
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Database links
- Entrez Gene: 6237 Human
- Omim: 165090 Human
- SwissProt: P10301 Human
- Unigene: 515536 Human
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Alternative names
- Oncogene RRAS antibody
- p23 antibody
- R Ras antibody
see all
Images
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Western blot - Anti-RRAS antibody (ab82356)All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HT29 (Human colon adenocarcinoma) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 5 : PC-3 whole cell lysate (ab3954)
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab82356 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RRAS antibody (ab82356)All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HT29 (Human colon adenocarcinoma) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 5 : PC-3 whole cell lysate (ab3954)
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
