Anti-GIT1 antibody (ab153958)
Key features and details
- Rabbit polyclonal to GIT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GIT1 antibody
See all GIT1 primary antibodies -
Description
Rabbit polyclonal to GIT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human GIT1 aa 375-631 (internal sequence).
Database link: Q9Y2X7 -
Positive control
- IHC-P: Human mixed ovarian cancer. ICC/IF: HeLa cells. WB: HepG2 whole cell lysate. Rat and mouse brain tissue lysate. HUVEC cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-GIT1 antibody (ab153958) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 84 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa labeling GIT1 with ab153958 at 1/500 dilution. Blue: Costaining with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody (ab153958)
Immunohistochemical analysis of paraffin-embedded Human mixed ovarian cancer tissue labeling GIT1 with ab153958 at 1/100 dilution.
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All lanes : Anti-GIT1 antibody (ab153958) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa7.5% SDS-PAGE
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Anti-GIT1 antibody (ab153958) at 1/500 dilution + HUVEV (Human umbilical vein endothelial cell line) whole cell extract at 30 µg
Predicted band size: 84 kDa7.5% SDS-PAGE