Mouse EGF ELISA Kit (ab234560)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.03 pg/ml
- Range: 1.56 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse EGF ELISA Kit
See all EGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 5 4.2% Inter-assay Sample n Mean SD CV% Urine 3 11% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.03 pg/ml -
Range
1.56 pg/ml - 150 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 97 92% - 102% Serum 103 101% - 105% Tissue Extracts 90 85% - 94% Cell culture media 97 93% - 101% EDTA Plasma 101 100% - 102% Cit plasma 114 101% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse EGF ELISA Kit (ab234560) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EGF protein in edta plasma, serum, tissue extracts, urine, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EGF with 1.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse EGF Capture Antibody 1 x 600µl 10X Mouse EGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse EGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate. -
Involvement in disease
Hypomagnesemia 4 -
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
Database links
- Entrez Gene: 13645 Mouse
- SwissProt: P01132 Mouse
- Unigene: 252481 Mouse
Images
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Other - Mouse EGF ELISA Kit (ab234560)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse EGF standard curve in Sample Diluent NS.The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Example of mouse EGF standard curve in 1X Cell Extraction Buffer PTR.The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse EGF standard curve in Sample Diluent 25BS.The EGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native EGF in mouse urine and kidney tissue supernatant samples.The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:10,000 and kidney supernatant 1:4. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 600,860 pg/mL in neat urine and 384.1 pg/mL in neat kidney tissue supernatant.
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Interpolated concentrations of native EGF in mouse kidney tissue extract based on a 400 µg/mL extract load.The concentrations of EGF were measured in duplicate and interpolated from the EGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 90.16 pg/mL in mouse kidney tissue extract.
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Interpolated concentrations of spike EGF in mouse serum and plasma samples.The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native EGF concentration was determined to be 7.456 pg/mL in neat serum, 63.90 pg/mL in neat plasma (citrate), and not detectable in neat plasma (EDTA). Native data is not shown.
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Mouse EGF Competitor Std Curve ComparisonStandard curve comparison between Mouse EGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity
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Sandwich ELISA - Mouse EGF ELISA Kit (ab234560)To learn more about the advantages of recombinant antibodies see here.
