Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GIT1 antibody [EPR10368] (ab171956)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10368] to GIT1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-GIT1 antibody [EPR10368]
See all GIT1 primary antibodies
Description

Rabbit monoclonal [EPR10368] to GIT1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GIT1 aa 550-650. The exact sequence is proprietary.
Database link: Q9Y2X7
Positive control
- WB: Human fetal brain, brain nuclear extract, HeLa and SH-SY5Y lysates. IHC-P: Human pancreas tissue, paraffin-embedded Human thyroid carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10368
Isotype

IgG
Research areas

Western blot - Anti-GIT1 antibody [EPR10368] (ab171956)

All lanes : Anti-GIT1 antibody [EPR10368] (ab171956) at 1/1000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human brain tissue nuclear fraction lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 84 kDa
Observed band size: 98 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab171956 observed at 98 kDa. Red - loading control ab8245 observed at 36 kDa.

ab171956 Anti-GIT1 antibody [EPR10368] was shown to specifically react with GIT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265230 (knockout cell lysate ab257965) was used. Wild-type and GIT1 knockout samples were subjected to SDS-PAGE. ab171956 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody [EPR10368] (ab171956)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GIT1 using ab171956 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GIT1 antibody [EPR10368] (ab171956)

All lanes : Anti-GIT1 antibody [EPR10368] (ab171956) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HeLa lysate
Lane 3 : SH-SY5Y lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody [EPR10368] (ab171956)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling GIT1 using ab171956 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-GIT1 antibody [EPR10368] (ab171956)

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