Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸ 10 µg 107 212 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [KB90] to CD11c
Suitable for: Flow Cyt, ICC
Reacts with: Mouse, Human

Product name

Anti-CD11c antibody [KB90]
See all CD11c primary antibodies
Description

Mouse monoclonal [KB90] to CD11c
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC	Mouse Human

See all applications and species data

Immunogen

Tissue, cells or virus corresponding to Human CD11c.

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Positive control
- ICC: Mouse splenocytes; THP-1 cells. Flow Cyt: Mouse splenocytes; human PBMCs.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

KB90
Isotype

IgG1
Research areas

Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

The negative controls are as follows:
-ve control 1: ab254183 at 1/50 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.
Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

Flow cytometric analysis of mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.
Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.
Anti-CD11c antibody [KB90] (ab254183)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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