Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD11c antibody [KB90] (ab254183)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [KB90] to CD11c
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD11c antibody [KB90]
    See all CD11c primary antibodies

  • Description

    Mouse monoclonal [KB90] to CD11c

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    ICC
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human CD11c.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC: Mouse splenocytes; THP-1 cells. Flow Cyt: Mouse splenocytes; human PBMCs.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)
    Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

  • Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)
    Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab254183 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

     

  • Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)
    Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

    Flow cytometric analysis of  mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

  • Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)
    Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

    Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

  • Anti-CD11c antibody [KB90] (ab254183)
    Anti-CD11c antibody [KB90] (ab254183)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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