Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
![Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-320488-anti-cd11c-antibody-epr21826-flow-cytometry.jpg "Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21826] to CD11c - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD11c antibody [EPR21826] - BSA and Azide free
See all CD11c primary antibodies -
Description
Rabbit monoclonal [EPR21826] to CD11c - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen tissue.
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General notes
Ab240558 is the carrier-free version of ab219799. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240558 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21826 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
Flow cytometric analysis of mouse primary splenocytes labeling CD11c with ab219799 at a 1/50 dilution.
Cells were stained with BV421 conjugated CD11b and rabbit IgG (Left panel) or CD11c (Right panel).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219799).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-319063-anti-cd11c-antibody-epr21826-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11c with ab219799 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on dendritic cells of mouse spleen (PMID: 20194631, PMID: 15795237) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab219799).
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![Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-3-benefits-of-recombinant-antibodies.png)
Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
