Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BNP antibody [EPR3735] - BSA and Azide free
    See all BNP primary antibodies

  • Description

    Rabbit monoclonal [EPR3735] to BNP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma and fetal heart lysate; ICC: Caco-2 cells; Flow Cyt: Caco-2 cells.

  • General notes

    ab247572 is the carrier-free version of ab92500 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
    Immunocytochemistry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

    This data was developed using ab92500, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 cells labelling BNP with ab92500 at 1/50 dilution (10.8 µg/mL), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplamic staining in Caco-2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

  • Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
    Western blot - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
    All lanes : Anti-BNP antibody [EPR3735] (ab92500) at 1/2000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92500, the same antibody clone in a different buffer formulation.

     

  • Flow Cytometry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
    Flow Cytometry - Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

    This data was developed using ab92500, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Caco-2 (Human colorectal adenocarcinoma epithelial cell) cells labelling BNP with ab92500 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)
    Anti-BNP antibody [EPR3735] - BSA and Azide free (ab247572)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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