Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD11c antibody [EPR21826] (ab219799)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21826] to CD11c
Suitable for: IHC-P, Flow Cyt
Reacts with: Mouse

Product name

Anti-CD11c antibody [EPR21826]
See all CD11c primary antibodies
Description

Rabbit monoclonal [EPR21826] to CD11c
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

IHC-P
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse spleen tissue. Flow Cyt: Mouse primary splenocytes.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Mouse
IHC-P	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21826
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] (ab219799)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11c with ab219799 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on dendritic cells of mouse spleen (PMID: 20194631, PMID: 15795237) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Flow Cytometry - Anti-CD11c antibody [EPR21826] (ab219799)

Flow cytometric analysis of mouse primary splenocytes labeling CD11c with ab219799 at a 1/50 dilution.

Cells were stained with BV421 conjugated CD11b and rabbit IgG (Left panel) or CD11c (Right panel).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Anti-CD11c antibody [EPR21826] (ab219799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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