Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [KB90] to CD11c - BSA and Azide free
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD11c antibody [KB90] - BSA and Azide free
    See all CD11c primary antibodies

  • Description

    Mouse monoclonal [KB90] to CD11c - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICCmore details
    Unsuitable for: IHC-P or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD11c.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC: Mouse splenocytes; THP-1 cells. Flow Cyt: Mouse splenocytes; human PBMCs.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255937 is the carrier-free version of ab254183. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)
    Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

  • Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)
    Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab254183 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

  • Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)
    Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

    Flow cytometric analysis of  mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

  • Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)
    Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

    Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

  • Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)
    Anti-CD11c antibody [KB90] - BSA and Azide free (ab255937)

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