Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GIT1 antibody [EPR10367] (ab156001)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10367] to GIT1
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-GIT1 antibody [EPR10367]
See all GIT1 primary antibodies
Description

Rabbit monoclonal [EPR10367] to GIT1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human GIT1 (UniProt Q9Y2X7).
Positive control
- WB: SH-SY5Y, HeLa, 293T, brain nuclear extract and fetal brain lysates. Flow Cyt: 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10367
Isotype

IgG
Research areas

Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)

All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human brain tissue nuclear fraction lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 84 kDa
Observed band size: 98 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab156001 observed at 98 kDa. Red - loading control ab8245 observed at 36 kDa.

ab156001 Anti-GIT1 antibody [EPR10367] was shown to specifically react with GIT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265230 (knockout cell lysate ab257965) was used. Wild-type and GIT1 knockout samples were subjected to SDS-PAGE. ab156001 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-GIT1 antibody [EPR10367] (ab156001)

Flow cytometric analysis of permeabilized 293T cells labeling GIT1 with ab156001 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)

All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Fetal brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa
Anti-GIT1 antibody [EPR10367] (ab156001)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com