Anti-GIT1 antibody [EPR10367] (ab156001)
![Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/ab156001-412750-anti-git1-antibody-epr10367-western-blot-human-knockout-lysate.jpg "Anti-GIT1 antibody [EPR10367] (ab156001)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10367] to GIT1
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-GIT1 antibody [EPR10367]
See all GIT1 primary antibodies -
Description
Rabbit monoclonal [EPR10367] to GIT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human GIT1 (UniProt Q9Y2X7).
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Positive control
- WB: SH-SY5Y, HeLa, 293T, brain nuclear extract and fetal brain lysates. Flow Cyt: 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10367 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human brain tissue nuclear fraction lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 84 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab156001 observed at 98 kDa. Red - loading control ab8245 observed at 36 kDa.
ab156001 Anti-GIT1 antibody [EPR10367] was shown to specifically react with GIT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265230 (knockout cell lysate ab257965) was used. Wild-type and GIT1 knockout samples were subjected to SDS-PAGE. ab156001 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/GIT1-Primary-antibodies-ab156001-2.jpg)
Flow Cytometry - Anti-GIT1 antibody [EPR10367] (ab156001)Flow cytometric analysis of permeabilized 293T cells labeling GIT1 with ab156001 at 1/100 dilution (red) compared to a rabbit IgG negative control (green). -
![Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/GIT1-Primary-antibodies-ab156001-1.jpg)
Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
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![Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/ab156001-4-benefits-of-recombinant-antibodies.png)
Anti-GIT1 antibody [EPR10367] (ab156001)
