FITC Anti-LOX 1 antibody [23C11] (ab81710)
![FITC Anti-LOX 1 antibody [23C11] (ab81710)](https://www.abcam.com/ps/products/81/ab81710/Images/ab81710-454449-fitc-anti-lox1-antibody-23c11-flow-cyt-human-pbmc.JPG "FITC Anti-LOX 1 antibody [23C11] (ab81710)")
Key features and details
- FITC Mouse monoclonal [23C11] to LOX 1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-LOX 1 antibody [23C11]
See all LOX 1 primary antibodies -
Description
FITC Mouse monoclonal [23C11] to LOX 1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab81710 reacts with LOX 1. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Fusion protein of extracellular domain (aa 71-273) of human LOX-1 with murine Fcgamma1 (LOX-1-muFc) produced in PEAK cells
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Positive control
- Human APCs
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General notes
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purification notes
Purified, 0.2 µm filtered solution. -
Primary antibody notes
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity. -
Clonality
Monoclonal -
Clone number
23C11 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)
Flow cytometric analysis showing expression of LOX1 on human PBMC cells at 10 µg/mL concentration.
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![Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)](https://www.abcam.com/ps/products/81/ab81710/Images/LOX-1-Primary-antibodies-ab81710-1.jpg)
Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)Overlay histogram showing A549 cells stained with ab81710 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81710, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
