Anti-LOX 1 antibody [EPR20750] (ab214427)
![Anti-LOX 1 antibody [EPR20750] (ab214427)](https://www.abcam.com/ps/products/214/ab214427/Images/ab214427-411346-AntiLOX-1-antibody-EPR20750-ab214427.png "Anti-LOX 1 antibody [EPR20750] (ab214427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20750] to LOX 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LOX 1 antibody [EPR20750]
See all LOX 1 primary antibodies -
Description
Rabbit monoclonal [EPR20750] to LOX 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours whole cell lysate; HUVEC untreated and treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 8 hours whole cell lysate; Human fetal vessel lysate; Mouse placenta lysate; Rat placenta lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20750 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LOX 1 antibody [EPR20750] (ab214427)All lanes : Anti-LOX 1 antibody [EPR20750] (ab214427) at 1/1000 dilution
Lane 1 : Human fetal vessel lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 31 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-LOX 1 antibody [EPR20750] (ab214427)](https://www.abcam.com/ps/products/214/ab214427/Images/ab214427-309842-anti-lox-1-antibody-epr20750-western-blot.jpg)
Western blot - Anti-LOX 1 antibody [EPR20750] (ab214427)All lanes : Anti-LOX 1 antibody [EPR20750] (ab214427) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, whole cell lysate
Lane 3 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : HUVEC treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 8 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile is consistent with what has been described in the literature (PMID: 10692464, PMID: 15016631).
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![Anti-LOX 1 antibody [EPR20750] (ab214427)](https://www.abcam.com/ps/products/214/ab214427/Images/ab214427-4-benefits-of-recombinant-antibodies.png)
Anti-LOX 1 antibody [EPR20750] (ab214427)
