Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873)

Key features and details

  • Mouse monoclonal [S39B-8] to GIT1 - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-GIT1 antibody [S39B-8] - C-terminal
    See all GIT1 primary antibodies

  • Description

    Mouse monoclonal [S39B-8] to GIT1 - C-terminal

  • Host species

    Mouse

  • Specificity

    Does not cross-react with GIT2.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment within Rat GIT1 aa 350 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9Z272

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873)
    Western blot - Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873)
    All lanes : Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873) at 1/1000 dilution

    Lane 1 : Rat brain membrane lysate
    Lane 2 : Blank: no lysate added

    Predicted band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873)
    Immunocytochemistry/ Immunofluorescence - Anti-GIT1 antibody [S39B-8] - C-terminal (ab240873)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling GIT1 with ab240873 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counterstain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).

    A:DAPI, B: Actin, C: GIT1, D: Merge.

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