Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP5H antibody [10G5AB9] (ab173006)

Key features and details

  • Mouse monoclonal [10G5AB9] to ATP5H
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-ATP5H antibody [10G5AB9]
    See all ATP5H primary antibodies

  • Description

    Mouse monoclonal [10G5AB9] to ATP5H

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml

    Lane 1 : Human Heart Homogenate at 20 µg
    Lane 2 : Rat Heart Homogenate at 20 µg
    Lane 3 : Mouse Heart Homogenate at 20 µg
    Lane 4 : Bovine Heart Homogenate at 15 µg
    Lane 5 : Hela cell lysate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).

     

    Fixation: Paraformaldehyde fixed (4%, 20 min).

    Used heated antigen retrieval for optimal signal.

    Permeabilization: Triton X-100 permeabilized (0.1%, 10min).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).

    Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.

    DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.

    The target protein locates to the mitochondria (red).

  • Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).

     

    Fixation: Paraformaldehyde fixed (4%, 15 min).

    Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).

    Blocking: 1% BSA /PBS (20 min).

    Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.

    Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.

    Washing: 2x 1% BSA/PBS.

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate at 20 µg
    Lane 2 : H4IIE cell lysate at 20 µg
    Lane 3 : MEF cell lysate at 20 µg
    Lane 4 : NIH-3T3 cell lysate at 20 µg
    Lane 5 : COS7 cell lysate at 10 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ATP5H antibody [10G5AB9] (ab173006)

© 2021 Astana Biomed Group LLP. All rights reserved.