Anti-WWOX (phospho Y33) antibody (ab193624)
Key features and details
- Rabbit polyclonal to WWOX (phospho Y33)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WWOX (phospho Y33) antibody
See all WWOX primary antibodies -
Description
Rabbit polyclonal to WWOX (phospho Y33) -
Host species
Rabbit -
Specificity
ab193624 detects endogenous levels of WWOX only when phosphorylated at tyrosine 33. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human WWOX (phospho Y33) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9NZC7 -
Positive control
- Extracts from HepG2 cells treated with PMA. Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193624 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WWOX (phospho Y33) antibody (ab193624)All lanes : Anti-WWOX (phospho Y33) antibody (ab193624) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells treated with PMA
Lane 2 : Extracts from HepG2 cells treated with PMA with antigen specific peptide
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX (phospho Y33) antibody (ab193624)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue sections labeling WWOX (phospho Y33) with ab193624 at a 1/50 dilution. Right image preincubated with blocking peptide.
