Anti-RASSF1 antibody [OTI8E4] (ab119423)
![Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-1.jpg "Anti-RASSF1 antibody [OTI8E4] (ab119423)")
Key features and details
- Mouse monoclonal [OTI8E4] to RASSF1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-RASSF1 antibody [OTI8E4]
See all RASSF1 primary antibodies -
Description
Mouse monoclonal [OTI8E4] to RASSF1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human RASSF1 aa 1-340. Protein produced in E.coli
Database link: NP_009113 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY RASSF1 cDNAIHC-P: Human breast, lung carcinoma, pancreas, thyroid carcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma,lymphoma, and tonsil tissuesFlow Cyt: COS7 cells transiently transfected with pCMV6-ENTRY RASSF1; Jurkat cells.
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General notes
The clone number has been updated from 8E4 to OTI8E4, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI8E4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-RASSF1 antibody [OTI8E4] (ab119423)All lanes : Anti-RASSF1 antibody [OTI8E4] (ab119423) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RASSF1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 39 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/ab119423-132730-anti-rassf1-antibody-oti8e4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Breast tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/ab119423-132729-anti-rassf1-antibody-oti8e4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/ab119423-132728-anti-rassf1-antibody-oti8e4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Thyroid carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Prostate carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Lymphoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/150 dilution staining RASSF1 in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/100 dilution staining RASSF1 in COS7 cells transiently transfected with pCMV6-ENTRY RASSF1 by Immunofluorescence. -
![Flow Cytometry - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-12.jpg)
Flow Cytometry - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/100 dilution staining RASSF1 in HEK293T cells transfected with either pCMV6-ENTRY RASSF1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry. -
![Flow Cytometry - Anti-RASSF1 antibody [OTI8E4] (ab119423)](https://www.abcam.com/ps/products/119/ab119423/Images/RASSF1-Primary-antibodies-ab119423-13.jpg)
Flow Cytometry - Anti-RASSF1 antibody [OTI8E4] (ab119423)ab119423 at 1/100 dilution staining RASSF1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
