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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)

Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10366(B)] to Tbx3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free
    See all Tbx3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10366(B)] to Tbx3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HeLa, C2C12, NIH/3T3, Rat-1 and L6 whole cell lysates.
  • General notes

    Ab249134 is the carrier-free version of ab154828. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249134 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10366(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis

Images

  • Western blot - Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)
    Western blot - Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)
    All lanes : Anti-Tbx3 antibody [EPR10366(B)] (ab154828) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : L6 (Rat skeletal muscle myoblast) whole cell lysate
    Lane 6 : Rat-1 (Rat embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    We are unsure how to define the extra bands.

  • Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)
    Anti-Tbx3 antibody [EPR10366(B)] - BSA and Azide free (ab249134)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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