Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)
![Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)](https://www.abcam.com/ps/products/256/ab256528/Images/ab256528-389453-anti-lrp5-antibody-epr22477-218-western-blot-human-knockout-lysate.jpg "Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22477-218] to LRP5 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free
See all LRP5 primary antibodies -
Description
Rabbit monoclonal [EPR22477-218] to LRP5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW260 and HEK293T cell lysates IP: 3T3-L1 whole cell lysate.
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General notes
ab256528 is the carrier-free version of ab223203 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab256528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22477-218 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)All lanes : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : LRP5 knockout HEK293T cell lysate
Lane 3 : SW620 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 179 kDa
Observed band size: 180-200 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab223203).
Lanes 1-3: Merged signal (red and green). Green - ab223203 observed at 180-200 kDa. Red - loading control ab7291 observed at 50 kDa.
ab223203 Anti-LRP5 antibody [EPR22477-218] was shown to specifically react with LRP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266618 (knockout cell lysate ab257202) was used. Wild-type and LRP5 knockout samples were subjected to SDS-PAGE. ab223203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)](https://www.abcam.com/ps/products/256/ab256528/Images/ab256528-1-IP1anti-lrp5-antibody-epr22477-218-immunoprecipitation-3t3l1-mouse.jpg)
Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)LRP5 was immunoprecipitated from 0.35 mg 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10µg with ab223203 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223203. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10µg.
Lane 2: ab223203 IP in 3T3-L1 differentiated adipocytes whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223203 in 3T3-L1 differentiated adipocytes whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
Differentiation procedure: https://www.abcam.com/protocols/differentiation-of-3t3-l1-cells-into-adipocyte-like-cells-protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223203).
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![Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)](https://www.abcam.com/ps/products/256/ab256528/Images/ab256528-3-benefits-of-recombinant-antibodies.png)
Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)
