Human Osteoprotegerin ELISA Kit (ab189580)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Osteoprotegerin ELISA Kit
See all Osteoprotegerin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6% Inter-assay Sample n Mean SD CV% Serum 3 9% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 100% - 103% Cell culture media 88 81% - 96% Hep Plasma 89 84% - 92% EDTA Plasma 98 86% - 104% Cit plasma 88 86% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow -
Product overview
Human Osteoprotegerin ELISA Kit (ab189580) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Osteoprotegerin with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Osteoprotegerin (OPG), also known as Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a 46kDa glycoprotein member of the tumor necrosis factor receptor superfamily. This cytokine is a homodimer expressed and secreted by multiple adult tissues such as the lung, liver, spleen, prostate, ovary, adrenal gland and bone marrow. It plays a critical function in bone homeostasis by acting as a decoy receptor for TNFSF11/RANKL and for the receptor activator of nuclear factor-kappa-B ligand. OPG therefore decreases bone resorption by inhibiting osteoclast differentiation and activation of osteoclast and by stimulating osteoclast apoptosis.
OPG deficiency has been found in Juvenile Paget disease, a rare autosomal recessive osteopathy that presents in infancy and early childhood characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures and deformities due to accelerated bone remodeling. OPG may also play a role in arterial calcification and high levels of OPG have been found to be an independent risk factor for cardiovascular disease and vascular mortality.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Osteoprotegerin Capture Antibody 1 x 600µl 10X Human Osteoprotegerin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Osteoprotegerin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. -
Tissue specificity
Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. -
Involvement in disease
Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal. -
Sequence similarities
Contains 2 death domains.
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated. Contains sialic acid residues.
The N-terminus is blocked. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MGC29565
- OCIF
- OPG
see all -
Database links
- Entrez Gene: 4982 Human
- Omim: 602643 Human
- SwissProt: O00300 Human
- Unigene: 81791 Human
Images
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Other - Human Osteoprotegerin ELISA Kit (ab189580)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Osteoprotegerin standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Osteoprotegerin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Ten individual healthy donors were evaluated for the presence of Osteoprotegerin in serum using this assay.Results were interpolated from the standard curve in Sample Diluent 25BS and corrected by sample dilution (1:2). The mean level of Osteoprotegerin is 378.8 pg/mL with a range of 256.5 – 584.5 pg/mL and a standard deviation of 106.7 pg/mL.
