Mouse VEGF R3 ELISA Kit (FLT4) (ab203367)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.97 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse VEGF R3 ELISA Kit (FLT4) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 4.59% Inter-assay Sample n Mean SD CV% Overall 3 7.14% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17.97 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85 81% - 88% Cell culture media 87 87% - 88% Hep Plasma 99 95% - 101% EDTA Plasma 100 96% - 104% Cit plasma 103 101% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow, Pig -
Product overview
Mouse VEGF R3 ELISA Kit (FLT4) (ab203367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF R3 (FLT4) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGF R3 (FLT4) with 17.97 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
The VEGF receptor family includes three members VEGF R1, VEGF R2, and VEGF R3 (FLT4). These receptors have a characteristic structure that includes 7 Ig-like domains in the extracellular domain and a cytoplasmic tyrosine kinase domain with a long kinase insert region. VEGF R3 is a tyrosine protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD. It plays an essential role in adult lymphangiogenesis, and in the development of the vascular network and cardiovascular system during embryonic development. VEGF R3 promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated VEGF R3 leads to enhanced production of VEGFC and VEGFA, creating a positive feedback loop that enhances FLT4 signaling.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse VEGF R3 Capture Antibody 1 x 600µl 10X Mouse VEGF R3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP 1 x 6ml Mouse VEGF R3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells. -
Cellular localization
Cell membrane; Single-pass type I membrane protein. -
Alternative names
- FLT 4
- FLT4
- FLT41
see all -
Database links
- Entrez Gene: 14257 Mouse
- SwissProt: P35917 Mouse
- Unigene: 3291 Mouse
Images
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Other - Mouse VEGF R3 ELISA Kit (FLT4) (ab203367)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of VEGF R3 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.The 1X dilution is 5% for mouse serum, and 2.5% for each of the plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Titration of mouse stimulated (100 ng/mL LPS + 50 ng/mL PMA, 3 days (+)) lung supernatant, and unstimulated (-) lung and liver cell culture supernatants within the working range of the assay.The 1X dilution is 50.0% for the lung supernatants, and 25.0% for the liver supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Sandwich ELISA - Mouse VEGF R3 ELISA Kit (FLT4) (ab203367)To learn more about the advantages of recombinant antibodies see here.
