Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
![Anti-alpha 1 Spectrin antibody [17C7] (ab11751)](https://www.abcam.com/ps/products/11/ab11751/Images/ab11751-352043-Anti-alpha-1-Spectrin-antibody-17C7-westernblot-lung.png "Anti-alpha 1 Spectrin antibody [17C7] (ab11751)")
Key features and details
- Mouse monoclonal [17C7] to alpha 1 Spectrin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-alpha 1 Spectrin antibody [17C7]
See all alpha 1 Spectrin primary antibodies -
Description
Mouse monoclonal [17C7] to alpha 1 Spectrin -
Host species
Mouse -
Specificity
ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
-
Positive control
- Erythroid spectrin in red blood cells and cerebellum.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
17C7 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)All lanes : Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/ml
Lane 1 : MW marker
Lane 2 : Human Lung lysate
Lane 3 : Mouse Lung lysate
Lane 4 : Rat Brain lysate
Lane 5 : Human Kidney lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP labeled goat anti-mouse IgG
Predicted band size: 280 kDa
-
![Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751)](https://www.abcam.com/ps/products/11/ab11751/Images/ab11751-19072-anti-alpha-1-spectrin-antibody-17c7-western-blot.jpg)
Western blot - Anti-alpha 1 Spectrin antibody [17C7] (ab11751) This image is courtesy of an Abreview submitted by Dr. Mahesh ShivananjappaAnti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg
Secondary
HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 280 kDa
Additional bands at: 200 kDa (possible cleavage fragment)
Exposure time: 1 hour
