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Mouse Myoglobin ELISA Kit (ab157722)

Key features and details

  • Sensitivity: 4.48 ng/ml
  • Range: 9.375 ng/ml - 300 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Myoglobin ELISA Kit
    See all Myoglobin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    4.48 ng/ml

  • Range

    9.375 ng/ml - 300 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s Myoglobin Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin in mouse samples. 


    In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


    Get better reproducibility in only 90 minutes with Mouse Myoglobin ELISA Kit (ab210965) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Properties

Images

  • Standard Curve
    Standard Curve
    Representative standard curve using ab157722 Myoglobin Mouse ELISA Kit

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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