Mouse Myoglobin ELISA Kit (ab210965)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.4 pg/ml
- Range: 15.62 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Myoglobin ELISA Kit
See all Myoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.1% Inter-assay Sample n Mean SD CV% Serum 3 4.8% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
9.4 pg/ml -
Range
15.62 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 110% - 123% Serum 88 76% - 99% Tissue Extracts 112 108% - 119% Hep Plasma 105 100% - 113% EDTA Plasma 100 95% - 106% Cit plasma 112 100% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Myoglobin ELISA Kit (ab210965) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Myoglobin with 9.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Myoglobin is an oxygen- and iron-binding protein involved in the transportation of oxygen within muscles. Upon muscle injury, myoglobin can also be found in the bloodstream.s
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Myoglobin Capture Antibody 1 x 600µl 10X Mouse Myoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Mouse Myoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
Sequence similarities
Belongs to the globin family. - Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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Database links
- Entrez Gene: 17189 Mouse
- SwissProt: P04247 Mouse
- Unigene: 404074 Mouse
Images
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Other - Mouse Myoglobin ELISA Kit (ab210965)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Myoglobin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Myoglobin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Myoglobin in mouse serum, plasma and cell culture supernatant samples.The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 37.5%, plasma (heparin) 20%, plasma (EDTA) 25% and heart supernatant 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 6.87 ng/mL in serum, 3.57 ng/mL in plasma (citrate), 6.35 ng/mL in plasma (heparin), 7.76 ng/mL in plasma (EDTA) and 4,187 ng/mL in heart supernatant.
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Interpolated concentrations of native Myoglobin in mouse tissue supernatant samples.The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: 1: 1,600 mouse heart tissue supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 4.19 µg/mL in mouse heart supernatant.
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Interpolated concentrations of native Myoglobin in mouse skeletal muscle based on a 300 ng/mL extract load and C2C12 extract based on a 125 µg/mL extract load.The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1.08 ng/mL in skeletal muscle extract and 0.67 ng/mL in C2C12 extract.
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Sandwich ELISA - Mouse Myoglobin ELISA Kit (ab210965)To learn more about the advantages of recombinant antibodies see here.
