Anti-WWOX antibody (ab226221)
Key features and details
- Rabbit polyclonal to WWOX
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-WWOX antibody
See all WWOX primary antibodies -
Description
Rabbit polyclonal to WWOX -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human WWOX aa 75-125. The exact sequence is proprietary. (NP_057457.1)
Database link: Q9NZC7 -
Positive control
- IP: Jurkat whole cell lysate. IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue.
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General notes
ab226221 has not performed satisfactorily when used for WB of WWOX in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of WWOX.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226221 was affinity purified using an epitope specific to WWOX immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-WWOX antibody (ab226221)
WWOX was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226221 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226221 at 0.4 µg/ml.
Lane 1: ab226221 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody (ab226221)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for WWOX using ab226221 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody (ab226221)Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for WWOX using ab226221 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
