Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23109-138] to WWOX - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-WWOX antibody [EPR23109-138] - BSA and Azide free
    See all WWOX primary antibodies

  • Description

    Rabbit monoclonal [EPR23109-138] to WWOX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, Human testis, HepG2, Mouse ovary, Mouse testis, human and mouse brain tissue lysate. IHC-P: Human breast carcinoma, Human testis, Human cerebrum and Mouse cerebrum tissues. Flow Cyt: MDA-MB-231 cells. IP: MCF7 and Mouse testis lysate.

  • General notes

    ab267348 is the carrier-free version of ab238144. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HNF1A with ab242140 at 36526 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas (PMID: 25793983). The section was incubated with ab242140 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242140).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling WWOX with ab238144 at 1/5000 dilution (0.10 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on mouse cerebrum (PMID: 17360458) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human cerebrum (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on Leydig cells in human testis (PMID: 16941225) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human breast carcinoma (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    WWOX was immunoprecipitated from 0.35 mg Mouse testis lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis lysate 10ug

    Lane 2: ab238144 IP in Mouse testis lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in Mouse testis lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    WWOX was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10µg

    Lane 2: ab238144 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Flow Cytometry - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Flow Cytometry - Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling WWOX with ab238144 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730/ Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MDA-MB-231（PMID: 19188760).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238144).

  • Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)
    Anti-WWOX antibody [EPR23109-138] - BSA and Azide free (ab267348)

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