Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WWOX antibody [EPR23109-138] (ab238144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23109-138] to WWOX
  • Suitable for: IP, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-WWOX antibody [EPR23109-138]
    See all WWOX primary antibodies

  • Description

    Rabbit monoclonal [EPR23109-138] to WWOX

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    IP
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, Human testis, HepG2, Mouse ovary, Mouse testis, human and mouse brain tissue lysate. IHC-P: Human breast carcinoma, Human testis, Human cerebrum and Mouse cerebrum tissues. Flow Cyt: MDA-MB-231 cells. IP: MCF7 and Mouse testis lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse ovary lysate
    Lane 4 : Mouse testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3:37 seconds; Lane 4:70 seconds.

  • Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: MDA-MB-231 (PMID: 19188760).

  • Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)
    All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa


    Exposure time: 37 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling WWOX with ab238144 at 1/5000 dilution (0.10 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on mouse cerebrum (PMID: 17360458) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human cerebrum (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on Leydig cells in human testis (PMID: 16941225) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human breast carcinoma (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

    WWOX was immunoprecipitated from 0.35 mg Mouse testis lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis lysate 10ug

    Lane 2: ab238144 IP in Mouse testis lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in Mouse testis lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

    WWOX was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab238144 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Flow Cytometry - Anti-WWOX antibody [EPR23109-138] (ab238144)
    Flow Cytometry - Anti-WWOX antibody [EPR23109-138] (ab238144)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling WWOX with ab238144 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MDA-MB-231（PMID: 19188760).

  • Anti-WWOX antibody [EPR23109-138] (ab238144)
    Anti-WWOX antibody [EPR23109-138] (ab238144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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