Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-WWOX antibody [EPR23109-138] (ab238144)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23109-138] to WWOX
Suitable for: IP, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-WWOX antibody [EPR23109-138]
See all WWOX primary antibodies
Description

Rabbit monoclonal [EPR23109-138] to WWOX
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Human
IP	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, Human testis, HepG2, Mouse ovary, Mouse testis, human and mouse brain tissue lysate. IHC-P: Human breast carcinoma, Human testis, Human cerebrum and Mouse cerebrum tissues. Flow Cyt: MDA-MB-231 cells. IP: MCF7 and Mouse testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23109-138
Isotype

IgG
Research areas

Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)

All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1 : Human testis lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse ovary lysate
Lane 4 : Mouse testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-3:37 seconds; Lane 4:70 seconds.
Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)

All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Negative control: MDA-MB-231 (PMID: 19188760).
Western blot - Anti-WWOX antibody [EPR23109-138] (ab238144)

All lanes : Anti-WWOX antibody [EPR23109-138] (ab238144) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa

Exposure time: 37 seconds

Blocking/diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling WWOX with ab238144 at 1/5000 dilution (0.10 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on mouse cerebrum (PMID: 17360458) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human cerebrum (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on Leydig cells in human testis (PMID: 16941225) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX antibody [EPR23109-138] (ab238144)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling WWOX with ab238144 at 1/1000 dilution (0.48 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on human breast carcinoma (PMID: 15692750) is observed. The section was incubated with ab238144 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

WWOX was immunoprecipitated from 0.35 mg Mouse testis lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse testis lysate 10ug

Lane 2: ab238144 IP in Mouse testis lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in Mouse testis lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunoprecipitation - Anti-WWOX antibody [EPR23109-138] (ab238144)

WWOX was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab238144 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2: ab238144 IP in MCF7 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238144 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Flow Cytometry - Anti-WWOX antibody [EPR23109-138] (ab238144)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling WWOX with ab238144 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: MDA-MB-231（PMID: 19188760).
Anti-WWOX antibody [EPR23109-138] (ab238144)

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