Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYP3A5 antibody [EPR4396] (ab108624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4396] to CYP3A5
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CYP3A5 antibody [EPR4396]
    See all CYP3A5 primary antibodies

  • Description

    Rabbit monoclonal [EPR4396] to CYP3A5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and colon tissue lysates. IHC-P: Human liver and kidney tissues. IP: Human fetal liver tissue lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Cytochrome P450 3A5

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/400 dilution (unpurified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    ab108624 (unpurified) at 1/20 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with unpurified ab108624 at 1/80. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    ab108624 (purified) at 1/50 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human kidney tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    All lanes : Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal colon tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human breast tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with purified ab108624 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Anti-CYP3A5 antibody [EPR4396] (ab108624)
    Anti-CYP3A5 antibody [EPR4396] (ab108624)

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