Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23963-116] to Wilms Tumor Protein - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free
    See all Wilms Tumor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR23963-116] to Wilms Tumor Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K-56 and NIH:OVCAR-3 whole cell lysates; Mouse testis tissue lysate; Rat testis tissue lysate. IHC-P: Human kidney, testis and ovarian carcinoma tissue; Mouse testis tissue; Rat testis tissue. ICC: OVCAR-3 cells.

  • General notes

    ab275970 is the carrier-free version of ab267377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275970 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunocytochemistry - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling Wilms tumor protein with ab267377 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in OVCAR-3 cells. Negative control: LnCap (PMID:11299720). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Wilms tumor protein with ab267377 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on human renal glomeruli (PMID:27922671). The section was incubated with ab267377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Western blot - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    All lanes : Anti-Wilms Tumor Protein antibody [EPR23963-116] (ab267377) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52,54 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab267377, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10438524).

    Negative control: LNCaP (PMID:11299720).

    Exposure time: 26 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Wilms tumor protein with ab267377 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on Sertoli cells of mouse testis (PMID:17229929). The section was incubated with ab267377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Western blot - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    All lanes : Anti-Wilms Tumor Protein antibody [EPR23963-116] (ab267377) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab267377, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10438524).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Wilms tumor protein with ab267377 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on Sertoli cells of human testis (PMID:17229929). The section was incubated with ab267377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling Wilms tumor protein with ab267377 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human ovarian carcinoma (PMID:16547468). The section was incubated with ab267377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

    This data was developed using ab267377, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Wilms tumor protein with ab267377 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on Sertoli cells of rat testis (PMID:17229929). The section was incubated with ab267377 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)
    Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free (ab275970)

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