Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)
![Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)](https://www.abcam.com/ps/products/249/ab249379/Images/ab249379-408122-anti-acid-phosphatase-acp1-antibody-epr9839-bsa-and-azide-free-western-blot-knockout-hek293-k562-hela-empty-human-lysates.jpg "Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free
See all Acid phosphatase/ACP1 primary antibodies -
Description
Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab249379 is the carrier-free version of ab166896 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249379 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Acid Phosphatase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9839 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : ACP1 knockout HEK-293 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 18 kDa
Observed band size: 18 kDaThis data was developed using ab166896, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab166896 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)](https://www.abcam.com/ps/products/249/ab249379/Images/ab249379-405818-anti-acid-phosphatase-acp1-antibody-epr9839-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 18 kDaThis data was developed using ab166896, the same antibody clone in a different buffer formulation.
-
![Flow Cytometry - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)](https://www.abcam.com/ps/products/249/ab249379/Images/ab249379-2-anti-acid-phosphatase-acp1-antibody-epr9839-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379) -
![Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)](https://www.abcam.com/ps/products/249/ab249379/Images/ab249379-1-anti-acid-phosphatase-acp1-antibody-epr9839-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)This data was developed using ab166896, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG. -
![Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)](https://www.abcam.com/ps/products/249/ab249379/Images/ab249379-5-benefits-of-recombinant-antibodies.png)
Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (ab249379)