Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16813] to Apolipoprotein CI/Apo-CI - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free
    See all Apolipoprotein CI/Apo-CI primary antibodies

  • Description

    Rabbit monoclonal [EPR16813] to Apolipoprotein CI/Apo-CI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab236616 is the carrier-free version of ab198288 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236616 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Apolipoprotein CI

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198288).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/800 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198288 at 1/800 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198288).

  • Flow Cytometry - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)
    Flow Cytometry - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/80 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) (ab172730) and cell without incubation with primary antibody and secondary antibody is blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198288).

  • Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)
    Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

    Immunoprecipitation analysis of Human plasma whole cell extract labeling Apolipoprotein CI/Apo-CI using ab198288 at 1/40 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198288 in Human plasma whole cell extract.
    Lane 1: Input: 10 μg Human plasma whole cell extract.
    Subsequent WB detection was performed using ab198288 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198288).

  • Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)
    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] - BSA and Azide free (ab236616)

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