Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9) - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free
See all PBK/SPK primary antibodies -
Description
Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate treated with Nocodazole. IHC-P: Human testis and rat spleen tissues. Flow Cyt: HeLa cells treated with Nocodazole.
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General notes
ab250790 is the carrier-free version of ab184953 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PBK
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2475(N) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution
Lane 1 : HeLa cell lysate treated with Nocodazole
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184953).
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Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184953).
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184953).
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Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK/SPK (phospho T9) peptide (blue) or non phosphopeptide (orange).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184953).
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