Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free (ab271927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16995] to Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) - BSA and Azide free
  • Suitable for: PepArr, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free
    See all Histone H2A+Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR16995] to Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: PepArr, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells.

  • General notes

    ab271927 is the carrier-free version of ab177186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177186).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177186).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177186).
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A+Histone H4 antibody [EPR16995] - BSA and Azide free (ab271927)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab177186 at 1/4000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177186).
  • Peptide Array - Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free (ab271927)
    Peptide Array - Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free (ab271927)

    ab177186 was tested in Peptide Array against human synthetic peptides to different Histone modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to histone H2A R3me1peptide (ab28756) and H4 R3me1peptide (ab17770), indicating that this antibody specifically recognises the histone H2A R3me1peptide (ab28756) and H4 R3me1peptide (ab17770).

    ab28756 - H2A R3me1
    ab17770 - H4 R3me1
    ab178994 - H2A unmod(1-30)
    ab179297 - H4 unmod(1-30)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177186).

  • Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free (ab271927)
    Anti-Histone H2A + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide free (ab271927)

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