Anti-Histone H2A (asymmetric di methyl R3) antibody (ab21574)
Key features and details
- Rabbit polyclonal to Histone H2A (asymmetric di methyl R3)
- Suitable for: IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2A (asymmetric di methyl R3) antibody
See all Histone H2A primary antibodies -
Description
Rabbit polyclonal to Histone H2A (asymmetric di methyl R3)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human Histone H2A aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab22398) -
Positive control
- IHC: Human normal colon FFPE tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanApplication Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.ELISA Use at an assay dependent concentration.This antibody gave a positive result in ELISA against the immunizing peptide (ab22398). For the following peptides it gave following results: the non-modified equivalent peptide (ab13186) - negative result, Histone H2A (symmetric di methyl R3)peptide(ab22399) - negative result. This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide. See figure below. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Notes IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.ELISA
Use at an assay dependent concentration.This antibody gave a positive result in ELISA against the immunizing peptide (ab22398). For the following peptides it gave following results: the non-modified equivalent peptide (ab13186) - negative result, Histone H2A (symmetric di methyl R3)peptide(ab22399) - negative result. This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide. See figure below. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Omim: 142720 Human
- Omim: 602786 Human
- Omim: 602797 Human
- SwissProt: P04908 Human
see all -
Alternative names
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (asymmetric di methyl R3) antibody (ab21574)
IHC image of ab21574 staining Histone H2A (asymmetric di methyl R3) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21574, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ELISA - Anti-Histone H2A (asymmetric di methyl R3) antibody (ab21574)This antibody gave a positive result in ELISA against the immunizing peptide ab22398 (blue line).
For the following peptides it gave following results:
Histone H2A (symmetric di methyl R3) peptide ab22399 (pink line) - negative result,
the non-modified equivalent peptide ab13186 (yellow line) - negative result.
This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide.
Datasheets and documents
References (1)
ab21574 has been referenced in 1 publication.
- Li Y et al. Arginine Methyltransferase 1 in the Nucleus Accumbens Regulates Behavioral Effects of Cocaine. J Neurosci 35:12890-902 (2015). WB ; Mouse . PubMed: 26377474
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (asymmetric di methyl R3) antibody (ab21574)
IHC image of ab21574 staining Histone H2A (asymmetric di methyl R3) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21574, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ELISA - Anti-Histone H2A (asymmetric di methyl R3) antibody (ab21574)
This antibody gave a positive result in ELISA against the immunizing peptide ab22398 (blue line).
For the following peptides it gave following results:
Histone H2A (symmetric di methyl R3) peptide ab22399 (pink line) - negative result,
the non-modified equivalent peptide ab13186 (yellow line) - negative result.
This indicates that it is specific for the Histone H2A (asymmetric di methyl R3) peptide.
