ChIP Kit (Histones, ChIP-seq) (ab270812)
Overview
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Product name
ChIP Kit (Histones, ChIP-seq) -
Product overview
ChIP Kit (Histones, ChIP-seq) (ab270812) provides a robust ChIP protocol suitable for the investigation of histone modifications within chromatin from cells and tissues. The protocol involves protein-DNA cross-linking with formaldehyde, followed by cell lysis and fragmentation of the cross-linked chromatin. The subsequent immunoprecipitation of chromatin is performed with an antibody (user supplied) specific to a target histone modification. Protein A magnetic beads are used to isolate the protein-DNA complexes of interest. The immunoprecipitated DNA is eluted and purified using unique IPure magnetic beads. The eluted DNA is ideal for ChIP-seq library preparation. Additionally, the kit includes a negative (IgG) and a positive (H3K4me3) control antibody and primer pairs amplifying H3K4me3 positive and negative loci.
Sample size:
Cells (100,000 cells to 1,000,000 cells per IP)
Tissues (1.5 mg to 5 mg of tissue per IP)
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Please refer to protocols. -
Components 24 tests 10 tests 5% BSA (DNA free) 1 x 144µl 1 x 60µl 5x ChIP Buffer C1 1 x 2.2ml 1 x 950µl Buffer C 1 x 1.6ml 1 x 700µl Carrier 1 x 64µl 1 x 28µl ChIP-seq grade antibody H3K4me3 1 x 8µg 1 x 8µg ChIP-seq grade GAPDH TSS primer pair 1 x 96µl 1 x 42µl ChIP-seq grade Myoglobin exon 2 primer pair 1 x 96µl 1 x 42µl ChIP-seq grade water 1 x 7ml 1 x 3ml Elution Buffer E1 1 x 3.4ml 1 x 1.5ml Elution Buffer E2 1 x 144µl 1 x 64µl Glycine 1 x 400µl 1 x 200µl Lysis Buffer L1 1 x 40ml 1 x 20ml Lysis Buffer L2 1 x 40ml 1 x 20ml Protease inhibitor cocktail 1 x 76µl 1 x 35µl Protein A-coated magnetic beads 1 x 480µl 1 x 200µl Purification Beads 1 x 320µl 1 x 140µl Rabbit IgG 1 x 8µg 1 x 8µg Shearing Buffer S1 1 x 7.2ml 1 x 3.6ml Wash Buffer 1 w/o iso-propanol 1 x 2ml 1 x 700µl Wash Buffer 2 w/o iso-propanol 1 x 2ml 1 x 700µl Wash Buffer W1 1 x 8.4ml 1 x 3.5ml Wash Buffer W2 1 x 8.4ml 1 x 3.5ml Wash Buffer W3 1 x 8.4ml 1 x 3.5ml Wash Buffer W4 1 x 8.4ml 1 x 3.5ml
